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aroma.affymetrix (version 2.2.0)

Analysis of large Affymetrix microarray data sets

Description

This package implements classes for files and sets of files for various Affymetrix file formats, e.g. AffymetrixCdfFile, AffymetrixCelFile, and AffymetrixCelSet. These are designed to be memory efficient but still being fast. The idea is to keep all data on file and only read data into memory when needed. Clever caching mechanisms are used to minimize the overhead of data IO. All of the above is hidden in the package API and for the developer (and the end user), the data is queried as if it lives in memory. With this design it is only the diskspace that limits the number of arrays that can be analyzed. To install, do: source("http://www.aroma-project.org/hbLite.R"); hbInstall("aroma.affymetrix").

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Version

Install

install.packages('aroma.affymetrix')

Monthly Downloads

608

Version

2.2.0

License

LGPL (>= 2.1)

Maintainer

Henrik Bengtsson

Last Published

September 3rd, 2011

Functions in aroma.affymetrix (2.2.0)

AffymetrixCnChpSet

The AffymetrixCnChpSet class
ChipEffectFile

The ChipEffectFile class
AdditiveCovariatesNormalization

The AdditiveCovariatesNormalization class
FirmaFile

The FirmaFile class
AffineSnpPlm

The AffineSnpPlm class
AlleleSummation

The AlleleSummation class
ExonProbeAffinityFile

The ExonProbeAffinityFile class
GenericReporter

The GenericReporter class
AvgSnpPlm

The AvgSnpPlm class
AffineCnPlm

The AffineCnPlm class
CnChipEffectSet

The CnChipEffectSet class
AffymetrixProbeTabFile

The AffymetrixProbeTabFile class
CnProbeAffinityFile

The CnProbeAffinityFile class
AffymetrixCelFile

The AffymetrixCelFile class
BasePositionNormalization

The BasePositionNormalization class
OpticalBackgroundCorrection

The OpticalBackgroundCorrection class
CnChipEffectFile

The CnChipEffectFile class
AffinePlm

The AffinePlm class
findUnitsTodo.UnitModel

Identifies non-fitted units
DChipSnpInformation

The DChipSnpInformation class
AvgCnPlm

The AvgCnPlm class
GcRmaBackgroundCorrection

The GcRmaBackgroundCorrection class
DChipDcpFile

The DChipDcpFile class
fromCdf.SnpInformation

Static method to define a genome information set from a CDF
QualityAssessmentFile

The QualityAssessmentFile class
QualityAssessmentModel

The QualityAssessmentModel class
BackgroundCorrection

The BackgroundCorrection class
GcContentNormalization

The GcContentNormalization class
AllelicCrosstalkCalibration

The AllelicCrosstalkCalibration class
AffymetrixFileSet

The AffymetrixFileSet class
MbeiSnpPlm

The MbeiSnpPlm class
DChipGenomeInformation

The DChipGenomeInformation class
AbstractProbeSequenceNormalization

The AbstractProbeSequenceNormalization class
ExonChipEffectSet

The ExonChipEffectSet class
ProbeLevelModel

The ProbeLevelModel class
HetLogAddSnpPlm

The HetLogAddSnpPlm class
extractMatrix.ParameterCelSet

Extract data as a matrix for a set of arrays
getCdf.AffymetrixCelFile

Gets the CDF structure for this CEL file
createFrom.AffymetrixCelFile

Creates a CEL file using another as a template
LinearModelProbeSequenceNormalization

The LinearModelProbeSequenceNormalization class
MbeiCnPlm

The MbeiCnPlm class
CnagCfhSet

The CnagCfhSet class
ChipEffectTransform

The ChipEffectTransform class
GcContentNormalization2

The GcContentNormalization2 class
CrlmmParametersSet

The CrlmmParametersSet class
CnagCfhFile

The CnagCfhFile class
FragmentEquivalentClassNormalization

The FragmentEquivalentClassNormalization class
BaseCountNormalization

The BaseCountNormalization class
ProbeLevelTransform3

The ProbeLevelTransform3 class
HetLogAddCnPlm

The HetLogAddCnPlm class
ExonChipEffectFile

The ExonChipEffectFile class
ScaleNormalization3

The ScaleNormalization3 class
MatSmoothing

The MatSmoothing class
AffymetrixCdfFile

The AffymetrixCdfFile class
ArrayExplorer

The ArrayExplorer class
RmaPlm

The RmaPlm class
CrlmmParametersFile

The CrlmmParametersFile class
AromaChipTypeAnnotationFile

The AromaChipTypeAnnotationFile class
SnpPlm

The SnpPlm interface class
UgpGenomeInformation

The UgpGenomeInformation class
MultiArrayUnitModel

The MultiArrayUnitModel class
DChipQuantileNormalization

The DChipQuantileNormalization class
WeightsFile

The WeightsFile class
averageQuantile.AffymetrixCelSet

Calculates the average empirical distribution across all samples
ResidualFile

The ResidualFile class
image270.AffymetrixCelFile

Displays all or a subset of the data spatially
AffymetrixFile

The abstract AffymetrixFile class
ChipEffectSet

The ChipEffectSet class
as.AffymetrixCnChpSet.AffymetrixCnChpSet

Coerce an object to an AffymetrixCnChpSet object
byChipType.AromaChipTypeAnnotationFile

Defines an AromaChipTypeAnnotationFile object by chip type
QualityAssessmentSet

The QualityAssessmentSet class
ChipEffectGroupMerge

The ChipEffectGroupMerge class
as.character.CnagCfhSet

Returns a short string describing the CNAG CFH set
SmoothMultiarrayModel

The SmoothMultiarrayModel class
MbeiPlm

The MbeiPlm class
as.CnagCfhSet.CnagCfhSet

Coerce an object to an CnagCfhSet object
getFullName.TransformReport

Gets the full name of the output data set
ParameterCelSet

The ParameterCelSet class
AffymetrixCelSetReporter

The AffymetrixCelSetReporter class
fit.SingleArrayUnitModel

Estimates the model parameters
getCellIndices.AffymetrixCdfFile

Gets the cell indices unit by unit
CnPlm

The CnPlm class
SmoothRmaModel

The SmoothRmaModel class
createMonocellCdf.AffymetrixCdfFile

Creates a mono-cell version of the CDF
Non-documented objects

Non-documented objects
bgAdjustGcrma.AffymetrixCelFile

Applies probe sequence based background correction to a CEL file
HetLogAddPlm

The HetLogAddPlm class
ResidualSet

The ResidualSet class
byChipType.DChipSnpInformation

Defines a DChipSnpInformation object by chip type
ExonRmaPlm

The ExonRmaPlm class
DChipCdfBinFile

The DChipCdfBinFile class
getAlias.Model

Gets the name alias for the model
FirmaModel

The FirmaModel class
ParameterCelFile

The ParameterCelFile class
MatNormalization

The MatNormalization class
FragmentLengthNormalization

The FragmentLengthNormalization class
DChipDcpSet

The DChipDcpSet class
AvgPlm

The AvgPlm class
FirmaSet

The FirmaSet class
AffymetrixFileSetReporter

The AffymetrixFileSetReporter class
SnpChipEffectSet

The SnpChipEffectSet class
as.character.AffymetrixCnChpSet

Returns a short string describing the set
getFitUnitGroupFunction.AffinePlm

Gets the low-level function that fits the PLM
getFitUnitGroupFunction.HetLogAddPlm

Gets the low-level function that fits the PLM
ReseqCrosstalkCalibration

The ReseqCrosstalkCalibration class
bgAdjustRma.AffymetrixCelFile

Applies normExp background correction to a CEL file
SpatialReporter

The SpatialReporter class
getAverageFile.CnagCfhSet

Calculates the mean and the standard deviation of the cell signal (intensity, standard deviation etc.) across the CFH set
getHeader.AromaChipTypeAnnotationFile

Gets the header of the annotation file
extractAffyBatch.AffymetrixCelSet

Extracts an in-memory AffyBatch object from the CEL set
convert.AffymetrixCdfFile

Converts a CDF into the same CDF but with another format
ProbeLevelTransform

The ProbeLevelTransform class
WeightsSet

The WeightsSet class
computeAffinities.AffymetrixCdfFile

Calculates probe affinities from sequence
ProbeAffinityFile

The ProbeAffinityFile class
byChipType.UgpGenomeInformation

Defines a UgpGenomeInformation object by chip type
getData.SnpInformation

Gets all or a subset of the genome information data
getCnNames.AffymetrixCdfFile

Gets the names of the CN units
Model

The Model class
as.character.DChipDcpSet

Returns a short string describing the DChip CHP set
ScaleNormalization

The ScaleNormalization class
as.AffymetrixCelSet.AffymetrixCelSet

Coerce an object to an AffymetrixCelSet object
getAlias.GenericReporter

Gets the alias of the report
getChipType.AffymetrixCelFile

Gets the chip type for this CEL file
NormExpBackgroundCorrection

The NormExpBackgroundCorrection class
fit.FirmaModel

Estimates the model parameters
aroma.affymetrix-package

Package aroma.affymetrix
RmaSnpPlm

The RmaSnpPlm class
getCdf.AffymetrixCelSet

Gets the CDF structure for this CEL set
getTags.GenericReporter

Gets the tags of the reporter
getUnitGroupCellMap.ChipEffectFile

Gets a (unit, group, cell) index map
bgAdjustOptical.AffymetrixCelFile

Applies optical background correction to a CEL file
getPath.Model

Gets the path of this model
process.QuantileNormalization

Normalizes the data set
getChipType.AffymetrixCelSet

Gets the chip type for this CEL set
SnpInformation

The SnpInformation class
getProbeAffinityFile.ProbeLevelModel

Gets the probe affinities for this model
createUniqueCdf.AffymetrixCdfFile

Creates a unique-cell version of the CDF
RmaBackgroundCorrection

The RmaBackgroundCorrection class
SnpChipEffectFile

The SnpChipEffectFile class
getPositions.GenomeInformation

Gets the physical positions for a set of units
nbrOfArrays.AffymetrixCnChpSet

Gets the number of arrays in the file set
calculateParametersGsb.AffymetrixCelSet

Computes parameters for adjustment of specific binding
SingleArrayUnitModel

The SingleArrayUnitModel class
calculateResidualSet.FirmaModel

Gets the set of residuals corresponding to the PLM
fit.SmoothMultiarrayModel

Fits the model for one chromosome across samples
allocateFromCdf.AffymetrixCelFile

Creates an empty CEL file from a template CDF
getRootPath.Model

Gets the root path of this model
bgAdjustGcrma.AffymetrixCelSet

Applies probe sequence based background correction to a set of CEL files
SnpChipEffectGroupMerge

The SnpChipEffectGroupMerge class
as.DChipDcpSet.DChipDcpSet

Coerce an object to an DChipDcpSet object
convertUnits.AffymetrixCdfFile

Gets and validates unit indices
getAM.ChipEffectFile

Gets the log-intensities and log-ratios of chip effects for two arrays
getImage.AffymetrixCelFile

Creates an RGB Image object from a CEL file
clearData.AffymetrixCelFile

Clears all or a subset of the fields in a CEL file
readUnits.AffymetrixCdfFile

Reads CDF data unit by unit
getWeights.QualityAssessmentModel

Calculates the weights from the robust fit to a probe-level model
byChipType.DChipGenomeInformation

Defines a DChipGenomeInformation object by chip type
readCdfDataFrame

Reads units (probesets) from an Affymetrix CDF file
getTags.Model

Gets the tags of the output data set
importFromDChip.CnChipEffectSet

Imports copy-number estimates from a dChip result file
extractMatrix.AffymetrixCelSet

Extract data as a matrix for a set of arrays
extractDataFrame.ParameterCelSet

Extract data as a data.frame for a set of arrays
compare.AffymetrixCdfFile

Checks if two AffymetrixCdfFile objects are equal
getOutputDataSet.Transform

Gets the transformed data set
isResequenceChip.AffymetrixCdfFile

Static method to check if a CDF is for a resequencing (CustomSeq) chip
getXAM.ChipEffectFile

Gets the physical position, log-intensities and log-ratios of chip effects for two arrays
fromDataFile.ChipEffectFile

Retrive an existing CEL file, or create from CDF template if missing
plotDensity.GenomeInformation

Plots the density of SNPs for a given chromosome
getAlleleProbePairs.AffymetrixCdfFile

Gets the indices of probepairs with the same pair of SNP nucleotides
setCdf.CnagCfhFile

Sets the CDF structure for this CEL file
UnitTypeScaleNormalization

The UnitTypeScaleNormalization class
getChipEffectSet.AlleleSummation

Gets the set of chip effects for this model
nbrOfSnps.AffymetrixCdfFile

Gets the number of SNPs
plotBoxplotStats.list

Plots a (merged or non-merged) list of boxplot.stats() elements
getChipType.GenomeInformation

Gets the chip type of this genome information set
TransformReport

The TransformReport class
fit.Model

Estimates the model parameters
UnitModel

The UnitModel class
nbrOfGroupsPerUnit.AffymetrixCdfFile

Gets the number of groups in each unit
getCellIndices.UnitModel

Gets the cell indices unit by unit
findUnitsTodo.FirmaModel

Identifies non-fitted units
updateUnits.AffymetrixCelFile

Updates CEL data unit by unit
GenomeInformation

The GenomeInformation class
getChipEffectSet.ProbeLevelModel

Gets the set of chip effects for this model
findUnitsTodo.ProbeLevelModel

Identifies non-fitted units
fromCdf.GenomeInformation

Static method to define a genome information set from a CDF
fromFile.AromaChipTypeAnnotationFile

Sets up an AromaChipTypeAnnotationFile
findByChipType.AromaChipTypeAnnotationFile

Locates an annotation file by its chip type
calculateBaseline.ChipEffectSet

Estimates the baseline signal chromosome by chromosome
byChipType.GenomeInformation

Static method to define a genome information set by chip type
findByChipType.AffymetrixCdfFile

Locates a CDF file from its chip type
getAM.ChipEffectSet

Gets the log-intensities and log-ratios of chip effects of the set relative to a reference chip effect file
getCdf.CnagCfhSet

Gets the CDF structure for this CFH set
getData.GenomeInformation

Gets all or a subset of the genome information data
readUnits.CnagCfhFile

Reads CEL data unit by unit
getBaseline.ChipEffectSet

Gets the baseline signals across chromosomes
smoothScatterMvsA.AffymetrixCelFile

Plots log-ratio versus log-intensity in a smooth scatter plot
setCdf.AffymetrixCelSet

Sets the CDF structure for this CEL set
getChipType.CnagCfhFile

Gets the chip type for this CEL file
getHeader.AffymetrixCdfFile

Gets the header of the CDF file
byChipType.SnpInformation

Static method to define a genome information set by chip type
getCdf.CnagCfhFile

Gets the CDF structure for this CEL file
process.GcContentNormalization

Normalizes the data set
getFitUnitGroupFunction.SingleArrayUnitModel

Static method to get the low-level function that fits the PLM
getFitUnitGroupFunction.FirmaModel

Static method to get the low-level function that fits the PLM
plotImage.AffymetrixCelFile

Displays a spatial plot of a CEL file
setRestructor.AffymetrixCdfFile

Specifies a function through which
extractExpressionSet.ChipEffectSet

Extracts an in-memory ExpressionSet object
fromFile.AffymetrixCelFile

Defines an AffymetrixCelFile object from a CEL file
setCdf.CnagCfhSet

Sets the CDF structure for this CFH set
getHeader.AffymetrixCelFile

Gets the header of the CEL file
process.AdditiveCovariatesNormalization

Normalizes the data set
getTags.TransformReport

Gets the tags of the output data set
process.DChipQuantileNormalization

Normalizes the data set
getFullName.Model

Gets the full name of the output set
fit.ProbeLevelModel

Estimates the model parameters
fromFile.CnagCfhFile

Defines an CnagCfhFile object from a CFH file
getAlleleProbePairs3.AffymetrixCdfFile

Gets the indices of probepairs with the same pair of SNP nucleotides
getDataSet.ArrayExplorer

Gets the data set
isSnpChip.AffymetrixCdfFile

Static method to check if a chip is a mapping (SNP) chip
nbrOfArrays.AffymetrixCelSet

Gets the number of arrays in the file set
allocateFromCdf.AromaUnitTabularBinaryFile

Creates an AromaUnitTabularBinaryFile mapping to a given CDF
process.AbstractProbeSequenceNormalization

Normalizes the data set
getFirmaSet.FirmaModel

Gets the set of FIRMA results for this model
normalizeQuantile.AffymetrixCelFile

Normalizes the probe intensities to a target empirical distribution
getAlleleCellPairs.AffymetrixCdfFile

Gets the cell indices of allele pairs
SnpProbeAffinityFile

The SnpProbeAffinityFile class
process.OpticalBackgroundCorrection

Performs background correction
fromFile.AffymetrixCdfFile

Defines an AffymetrixCdfFile object from a CDF file
setAlias.Model

Sets the name alias for the model
groupUnitsByDimension.AffymetrixCdfFile

Groups units by dimensions
process.GcRmaBackgroundCorrection

Performs background correction
getSnpNames.AffymetrixCdfFile

Gets the names of the SNP units
process.SpatialReporter

Generates image files, scripts and dynamic pages for the explorer
getCdf.Model

Gets the CDF structure for this model
getDataSet.AffymetrixCelSetReporter

Gets the data set
getFitUnitGroupFunction.MbeiPlm

Gets the low-level function that fits the PLM
getAverageFile.AffymetrixCelSet

Calculates the mean and the standard deviation of the cell signal (intensity, standard deviation etc.) across the CEL set
getFitUnitGroupFunction.AvgPlm

Gets the low-level function that fits the PLM
plotDensity.AffymetrixCelSet

Plots the densities of all samples
plotMvsX.AffymetrixCelFile

Plots log-ratio versus another variable in a scatter plot
pdInfo2Cdf

Generates an Affymetrix CDF file from a Platform Design (PD) package and a auxillary CEL file for the same chip type
getHeader.CnagCfhFile

Gets the header of the CEL file
plotMvsA.AffymetrixCelFile

Plots log-ratio versus log-intensity in a scatter plot
readRawData.AffymetrixCelFile

Gets all or a subset of the fields in a CEL file
process.ArrayExplorer

Generates image files, scripts and dynamic pages for the explorer
process.RmaBackgroundCorrection

Performs background correction
getUnitIndices.GenomeInformation

Gets unit indices ordered along the chromosome
getUnitTypes.AffymetrixCdfFile

Gets the types of a set of units
LimmaBackgroundCorrection

The LimmaBackgroundCorrection class
nbrOfArrays.CnagCfhSet

Gets the number of arrays in the file set
process.ChipEffectGroupMerge

Normalizes the data set
setArrays.ArrayExplorer

Sets the arrays
QuantileNormalization

The QuantileNormalization class
getGenomeInformation.AffymetrixCdfFile

Gets genome information for this chip type
getName.TransformReport

Gets the name of the output data set
process.ScaleNormalization3

Normalizes the data set
setTags.Model

Sets the tags to be appended
process.ScaleNormalization

Normalizes the data set
writeImage.AffymetrixCelFile

Writes a spatial image of the signals in the CEL file
readUnitsByQuartets.AffymetrixCdfFile

Gets the cell quartets for each base position
importFromDChip.AffymetrixCelSet

Imports dChip-exported CEL files
getChipType.SnpInformation

Gets the chip type of this genome information set
verify.SnpInformation

Verifies the correctness of the underlying file
getName.GenericReporter

Gets the name of the explorer
process.FragmentLengthNormalization

Normalizes the data set
getFitUnitGroupFunction.RmaPlm

Gets the low-level function that fits the PLM
isPm.AffymetrixCdfFile

Checks which cells (probes) are PMs and not
readUnits.AffymetrixCelFile

Reads CEL data unit by unit
process.MatNormalization

Normalizes the data set
getXAM.ChipEffectSet

Gets the physical position, log-intensities and log-ratios of chip effects for two arrays
importFromApt.CnChipEffectSet

Imports copy-number estimates from an APT summary file
getName.Model

Gets the name of the output data set
isDuplicated.AffymetrixCelSet

Identifies duplicated CEL files
getFitUnitGroupFunction.ExonRmaPlm

Gets the low-level function that fits the Exon PLM
getInputDataSet.TransformReport

Gets the source data set
setAlias.GenericReporter

Sets the alias of the report
getTimestamp.AffymetrixCelFile

Gets the timestamp in the CEL header
getUnitIntensities.AffymetrixCelSet

Gets cell signals for a subset of units and a subset of arrays
process.GenericReporter

Generates report
process.BackgroundCorrection

Processes the data set
normalizeQuantile.AffymetrixCelSet

Normalizes samples to have the same empirical distribution
process.MatSmoothing

Processes the data set
process.ReseqCrosstalkCalibration

Calibrates the data set
getResiduals.QualityAssessmentModel

Calculates the residuals from a probe-level model
getUnitNames.AffymetrixCdfFile

Gets the names of each unit
readUnits.MultiArrayUnitModel

Reads data unit by unit
getPath.TransformReport

Gets the path of the output data set
process.AllelicCrosstalkCalibration

Calibrates the data set
process.FragmentEquivalentClassNormalization

Normalizes the data set
RmaCnPlm

The RmaCnPlm class
process.UnitTypeScaleNormalization

Normalizes the data set
readUnits.SingleArrayUnitModel

Reads data unit by unit
process.LimmaBackgroundCorrection

Performs background correction
verify.GenomeInformation

Verifies the correctness of the underlying file
as.character.AffymetrixCelSet

Returns a short string describing the Affymetrix CEL set
bgAdjustOptical.AffymetrixCelSet

Applies optical background correction to a set of CEL files
bgAdjustRma.AffymetrixCelSet

Applies RMA background correction to a set of CEL files
getIntensities.AffymetrixCelSet

Gets cell intensities from a set of cells and a set of arrays
getOutputDataSet.TransformReport

Gets the transformed data set
plotDensity.AffymetrixCelFile

Plots the density of the probe signals on the array
writeCdf.AffyGenePDInfo

Generates an Affymetrix CDF file from a Platform Design (PD) package
AffymetrixCelSet

The AffymetrixCelSet class
AffymetrixCelSetTuple

The AffymetrixCelSetTuple class
as.AffymetrixFileSet.AffymetrixFileSet

Coerce an object to an AffymetrixFileSet object
byPath.AffymetrixFileSet

Defines an AffymetrixFileSet object by searching for Affymetrix files
bpmapCluster2Cdf

Creates a CDF from tiling-array BPMAP file
fitQuantileNormFcn.AffymetrixCelFile

Fits quantile normalization functions for the arrays in the data set
getDataSet.Model

Gets the input data set for this model
getFitUnitGroupFunction.MultiArrayUnitModel

Static method to get the low-level function that fits the PLM
isDuplicated.CnagCfhSet

Identifies duplicated CFH files
nbrOfArrays.DChipDcpSet

Gets the number of arrays in the file set
setCdf.AffymetrixCelFile

Sets the CDF structure for this CEL file