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Imports qPCR data from RDML v1.1 format files (Lefever et al. 2009) and transforms it to the appropriate format of the qpcR package (Ritz et al. 2008, Spiess et al. 2008) or chipPCR package. RDML (Real-time PCR Data Markup Language) is the recommended file format element in the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines (Bustin et al. 2009). Data can be imported to the one table format (flat) or list of tables spited by PCR targets and samples types. Tables consist of 'Cycles' in the first column and fluorescence data of the samples in the remaining columns for qPCR data. For melting data tables consist of 'Temperature' in the first column and fluorescence data of the samples in the remaining columns.

Installation

You can install the latest development version of the code using the devtools R package.

# Install devtools, if you haven't already.
install.packages("devtools")

library(devtools)
install_github("RDML", "kablag")

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Version

Install

install.packages('RDML')

Monthly Downloads

259

Version

0.9-1

License

GPL (>= 2)

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Maintainer

Konstantin A Blagodatskikh

Last Published

July 22nd, 2015

Functions in RDML (0.9-1)

cdnaSynthesisMethodType

cdnaSynthesisMethodType R6 class.
documentationType

documentationType R6 class.
xRefType

xRefType R6 class.
new

Creates new instance of RDML class object
adpsType

adpsType R6 class.
runType

runType R6 class.
RDML.AsDendrogram

Represents structure of RDML file as dendrogram
mdpsType

mdpsType R6 class.
experimentType

experimentType R6 class.
baseTemperatureType

baseTemperatureType R6 class.
enumType

enumType R6 class.
stepType

stepType R6 class.
experimenterType

experimenterType R6 class.
pcrFormatType

pcrFormatType R6 class.
sequencesType

sequencesType R6 class.
pauseType

pauseType R6 class.
templateQuantityType

templateQuantityType R6 class.
rdmlBaseType

Base R6 class for RDML package.
RDML

R6 class RDML -- contains methods to read and overview fluorescence data from RDML v1.1 and v1.2 format files
dataCollectionSoftwareType

dataCollectionSoftwareType R6 class.
sampleType

sampleType R6 class.
dyeType

dyeType R6 class.
temperatureType

temperatureType R6 class.
idReferencesType

idReferencesType R6 class.
AsTable

RDML$AsTable() wrapper
RDML.SetFData

Sets fluorescence data vectors to RDML object
quantityUnitType

quantityUnitType R6 class.
GetFData

RDML$GetFData() wrapper
targetType

targetType R6 class.
thermalCyclingConditionsType

thermalCyclingConditionsType R6 class.
RDML.AsTable

Represents fields of RDML object as data.frame
gradientType

gradientType R6 class.
quantityType

quantityType R6 class.
sampleTypeType

sampleTypeType R6 class.
oligoType

oligoType R6 class.
cqDetectionMethodType

cqDetectionMethodType R6 class.
measureType

measureType R6 class.
idType

idType R6 class.
rdmlIdType

rdmlIdType R6 class.
reactIdType

reactIdType R6 class.
MergeRDMLs

Merges RDML objects
nucleotideType

nucleotideType R6 class.
primingMethodType

primingMethodType R6 class.
AsDendrogram

RDML$AsDendrogram() wrapper
loopType

loopType R6 class.
commercialAssayType

commercialAssayType R6 class.
lidOpenType

lidOpenType R6 class.
dataType

dataType R6 class.
labelFormatType

labelFormatType R6 class.
SetFData

RDML$SetFData() wrapper
targetTypeType

targetTypeType R6 class.
annotationType

annotationType R6 class.
RDML.GetFData

Gets fluorescence data vectors from RDML object
reactType

reactType R6 class.