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FourCSeq (version 1.6.2)

Package analyse 4C sequencing data

Description

FourCSeq is an R package dedicated to the analysis of (multiplexed) 4C sequencing data. The package provides a pipeline to detect specific interactions between DNA elements and identify differential interactions between conditions. The statistical analysis in R starts with individual bam files for each sample as inputs. To obtain these files, the package contains a python script (extdata/python/demultiplex.py) to demultiplex libraries and trim off primer sequences. With a standard alignment software the required bam files can be then be generated.

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Version

Version

1.6.2

License

GPL (>= 3)

Maintainer

Felix A Klein

Last Published

February 15th, 2017

Functions in FourCSeq (1.6.2)

FourC

FourC-class
addFragments

Add the restriction fragment information
addPeaks

Add peaks based on z-scores and adjusted p-values
combineFragEnds

Combine the counts of both fragment ends.
getDistAroundVp

getDistAroundVp
smoothHitPerCent

Smooth the hits of neighboring fragments
plotZScores

Plot z-score results.
plotScatter

plotScatter
plotNormalizationFactors

Plot the estimated normalization factors.
fcf

FourC object with z-scores
getZScores

Calculate z-scores using the residuals of the general trend fit.
plotDifferences

Plot differences
getReferenceSeq

Function to read reference sequences
distFitMonotone

Fit the distance dependency
plotFits

Plot fit results.
smoothCounts

Smooth the counts of neighboring fragments
getNormalizationFactors

Get normalization factors for each fragment
writeTrackFiles

Write track files of an selected assay
fc

FourC object with counts
distFitMonotoneSymmetric

Fit the distance dependency
getDifferences

Detect differences
countFragmentOverlaps

Count fragment overlaps
addViewpointFrags

Add the information of the viewpoint fragments
countFragmentOverlapsSecondCutter

Count fragment overlaps when sequencing was performed from the second cutting site
normalizeRPM

Normalize count data to rpm
findViewpointFragments

Find the fragments to which the viewpoint primers map.
getAllResults

FourCSeq analysis results