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SGSeq (version 1.0.6)

Prediction, quantification and visualization of alternative transcript events from RNA-seq data

Description

RNA-seq data are informative for the analysis of known and novel transcript isoforms. While the short length of RNA-seq reads limits the ability to predict and quantify full-length transcripts, short read data are well suited for the analysis of individual alternative transcripts events (e.g. inclusion or skipping of a cassette exon). The SGSeq package enables the prediction, quantification and visualization of alternative transcript events from BAM files.

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Version

Version

1.0.6

License

Artistic-2.0

Maintainer

Leonard Goldstein

Last Published

February 15th, 2017

Functions in SGSeq (1.0.6)

exportFeatures

Export to BED format
analyzeFeatures

Analysis of splice graph features from BAM files
convertToSGFeatures

Convert transcript features to splice graph features
convertToTxFeatures

Convert to TxFeatures object
filterFeatures

Filter predicted features
assays

Accessing and replacing assay data
exonCompatible

Compatible fragment counts for exons
annotate

Annotation with respect to transcript features
annotateTxVariants

Annotate transcript variants in terms of canonical events
analyzeVariants

Analysis of transcript variants
filterTerminalExons

Filter terminal exons that share splice sites with internal exons
findTxVariants

Find transcript variants from splice graph
getTxVariantCounts

Representative counts and frequency estimates for transcript variants
getBamInfo

Obtain alignment information from BAM files
findOverlapsRanges

Modified findOverlaps function for IRanges, IRangesList objects
gr

Example region of interest
junctionCompatible

Compatible fragment counts for splice junctions
getSGFeatureCounts

Compatible counts for splice graph features from BAM files
mergeTxFeatures

Merge redundant features
getSGFeatureCountsPerSample

Compatible fragment counts for splice graph features
plotVariants

Plot splice graph and heatmap of variant frequencies
predictTxFeaturesPerSample

Identification of splice junctions and exons from BAM file
predictCandidatesInternal

Identify candidate internal exons
txv

Example transcript variants
predictTxFeaturesRanges

Identification of splice junctions and exons for a given chromosome and strand
TxSegments

Constructor function for S4 class TxSegments
predictSpliced

IRanges-based identification of splice junctions and exons
predictTxFeatures

Transcript feature prediction from BAM files
removeExonsIsolated

Remove exons with no flanking splice junctions
sgf

Example splice graph features
TxVariantCounts

Constructor function for S4 class SGFeatureCounts
TxVariants

Constructor function for S4 class TxVariants
sgfc

Example splice graph feature counts
predictExonsTerminal

Identify terminal exons
SGFeatureCounts

Constructor function for S4 class SGFeatureCounts
predictJunctions

Identify splice junctions
plotFeatures

Plot splice graph and heatmap of expression values
plotSpliceGraph

Plot splice graph
slots

Accessing and replacing column slots
splicesiteOverlap

Compatible fragment counts for splice sites
txvc

Example transcript variant counts
predictCandidatesTerminal

Identify candidate terminal exons
SGFeatures

Constructor function for S4 class SGFeatures
predictExonsInternal

Identify internal exons
TxFeatures

Constructor function for S4 class TxFeatures
si

Example sample information
txf

Example transcript features